Analysis Note

ControlMouse brain tissue lysate

Application

Immunohistochemistry Analysis: 1 µg/mL from a representative lot detected TRIM5 delta in mouse brain tissue.

Anti-TRIM5 delta Antibody is a highly specific rabbit polyclonal antibody, that targets Tripartite motif-containing protein 5 & has been tested in western blotting & IHC.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tripartite motif-containing protein 5 (TRIM5), also known as the RING finger protein 88 (RNF88), is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. The TRIM5 delta isoform has been shown to colocalize with BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells serving as a scaffold for the assembly of endogenous BTBD proteins.

Immunogen

KLH-conjugated linear peptide corresponding to human TRIM5 delta.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected TRIM5 delta in 15 µg of mouse brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~25 kDa observed. Uniprot describes 6 isoforms ranging between ~30 kDa and ~56 kDa